牛昱宇博士

“國(guó)家高層次人才”特聘教授項(xiàng)目入選者、科技部重點(diǎn)研發(fā)計(jì)劃項(xiàng)目首席科學(xué)家

Yuyu Niu, Ph.D.

Dean & Professor of Faculty of Life Science and Technology, KUST

Associate Director and Professor of State Key Laboratory of Primate Biomedical Research

一、工作學(xué)習(xí)經(jīng)歷

2022年-今，昆明理工大學(xué)，副校長(zhǎng)

2019年-2022年，昆明理工大學(xué)生命科學(xué)與技術(shù)學(xué)院，教授，院長(zhǎng)

2014年-，昆明理工大學(xué)靈長(zhǎng)類轉(zhuǎn)化醫(yī)學(xué)研究院，教授

2007年-2014年，中國(guó)科學(xué)院昆明動(dòng)物研究所，副研究員

2006年-2008年，德國(guó)柏林野生與籠養(yǎng)動(dòng)物研究所，訪問學(xué)者

2001年-2007年，中國(guó)科學(xué)院昆明動(dòng)物研究所，博士

1995年-1999年，云南師范大學(xué)生命科學(xué)系，本科

二、研究領(lǐng)域

靈長(zhǎng)類基因編輯；靈長(zhǎng)類胚胎發(fā)育與疾病

Gene editing, Primate embryonic development

早期胚胎發(fā)育是哺乳動(dòng)物生命發(fā)生的重要階段，這一時(shí)期的關(guān)鍵生物學(xué)事件，如合子基因啟動(dòng)，表觀遺傳維持與重建，胚層分化和器官形成等等，是發(fā)育生物學(xué)的重要基礎(chǔ)理論，又和人類健康和疾病密切相關(guān)。然而，我們對(duì)其中的諸多科學(xué)問題卻知之甚少。本實(shí)驗(yàn)室結(jié)合靈長(zhǎng)類早期胚胎（體外受精，單性生殖和體細(xì)胞核移植）培養(yǎng)，基因編輯，干細(xì)胞與示蹤，單細(xì)胞組學(xué)和靈長(zhǎng)類疾病動(dòng)物模型等技術(shù)體系，系統(tǒng)研究靈長(zhǎng)類細(xì)胞多能性的維持與重建，表觀遺傳維持與胚層分化的關(guān)系，退行性疾病的早期診斷和干預(yù)等等重要科學(xué)問題，以期揭示人類生命發(fā)生和疾病形成的奧秘。

Research in Yuyu Niu's laboratory is focused on early embryo development and reprogramming, particularly preimplantation embryo development which play an important role in cell differentiation and organ initiation, and therefore have a significant on human disease. Over the last decade, Dr. Niu has made significant contributions to non-human primate genetic engineering and early embryo development that include: 1) the first transgenic rhesus monkey in China (PNAS 2010); 2) the first monkey model with genome engineered by precision gene-targeting methods (Cell 2014; Cell 2017); 3) the continuous development of monkey embryos in vitro beyond early gastrulation and to 20 days post fertilization (Science 2019). Built on the strength in non-human primate research, the Yuyu Niu's laboratory has expanded its capability to use a variety of state-of-the-art techniques, including single cell transcriptomics, cell lineage tracing, stem cells, somatic cell nuclear transfer and CRISPR/Cas9-based genomic editing tounderstand the molecularevents at the beginning of primate life and cell reprogramming. In addition, the lab has studied on mechanism and biomarker of neurodegeneration diseases, such as Parkinson’s disease and Amyotrophic lateral sclerosis.

三、開設(shè)課程

細(xì)胞生物學(xué)、發(fā)育生物學(xué)

四、代表性論文（近5年）

1.Kang Y, D. S.,Zeng Y, Wang F, Yang P, Yang Z, Pu Y, Li Z, Chen X, Tian B, Si W, Ji W*, Niu Y*. Cloningand base editingof GFP transgenic rhesus monkey and off-target analysis.Science Advances,doi:10.1126/sciadv.abo3123(2022). (* co-corresponding author)

2.Zhu X, G. Y., Chu C, Liu D, Duan K, Yin Y, Si C, Kang Y, Yao J, Du X, Li J, Zhao S, Ai Z, Zhu Q, Ji W*, Niu Y*, Li T*. BRN2 as a key gene drives the early primate telencephalon developmentScience Advances, doi:10.1126/sciadv.abl7263 (2022). (* co-corresponding author)

3.Zhong T, W. J., Wu K, Chen L, Zhao F, Pei Y, Wang Y, Zhang H, Wu Z, Huang Y, Li T, Wang L, Chen Y, Ji W, Zhang Y, Li G*, Niu Y*. Longitudinal brain atlases of early developing cynomolgus macaques from birth to 48 months of age.Neuroimage247, 118799, doi:10.1016/j.neuroimage.2021.118799 (2022). (* co-corresponding author)

4.Yang R, G. A., Kang Y, Si C, Chu C, Zheng Y, Chen Z, Gruber PJ, Xiao Y, Zhou C, Witman N, Eroglu E, Leung CY, Chen Y, Fu J, Ji W*, Lanner F*, Niu Y*, Chien KR*. Amnion signals are essential for mesoderm formation in primates.Nature communications12, 5126 (2021). (* co-corresponding author)

5.Tan T*, W. J., Si C, Dai S, Zhang Y, Sun N, Zhang E, Shao H, Si W, Yang P, Wang H, Chen Z, Zhu R, Kang Y, Hernandez-Benitez R, Martinez Martinez L, Nu?ez Delicado E, Berggren WT, Schwarz M, Ai Z, Li T, Rodriguez Esteban C, Ji W*, Niu Y*, Izpisua Belmonte JC*. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.Cell184, 2020-2032 e2014, doi:10.1016/j.cell.(2021). (* co-corresponding author)

6.Chu C, Z. W*. , Kang Y, Si C, Ji W*, Niu Y*, Zhang Y*. Analysis of developmental imprinting dynamics in primates using SNP-free methods to identify imprinting defects in cloned placenta.Developmental cell56, 2826-2840. e2827 (2021). (* co-corresponding author)

7.Chen ZZ, W. J., Kang Y, Yang QY, Gu XY, Zhi DL, Yan L, Long CZ, Shen B*, Niu YY*. PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys.Zoological Research42, 469 (2021). (* co-corresponding author)

8.Chen C, J. W., Niu Y*. Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research.Trends in Biotechnology39(12):1332-1342 (2021).

9.Wang F, Z. W., Yang Q, Kang Y, Fan Y, Wei J, Liu Z, Dai S, Li H, Li Z, Xu L, Chu C, Qu J, Si C, Ji W*, Liu GH*, Long C*, Niu Y*. Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing.Protein & cell11, 809-824 (2020). (* co-corresponding author)

10.Li B, N. Y*., Ji W*, Dong Y*. Strategies for the CRISPR-based therapeutics.Trends in pharmacological sciences41, 55-65 (2020). (* co-corresponding author)

11.Wang D, N. Y*., Ren L, Kang Y, Tai PWL, Si C, Mendonca CA, Ma H, Gao G, Ji W. Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus.Advanced Science6, 1900440 (2019). (* co-firstauthor)

12.Niu Y*, S. N., Li C, Lei Y, Huang Z, Wu J, Si C, Dai X, Liu C, Wei J, Liu L, Feng S, Kang Y, Si W, Wang H, Zhang E, Zhao L, Li Z, Luo X, Cui G, Peng G, Izpisúa Belmonte JC*, Ji W*, Tan T*. Dissecting primate early post-implantation development using long-term in vitro embryo culture.Science366 (2019). (* co-corresponding author)

13.Kang Y, C. C., Wang F, Niu Y*. CRISPR/Cas9-mediated genome editing in nonhuman primates.Disease models & mechanisms12, dmm039982 (2019).

五、主持項(xiàng)目（正在執(zhí)行）

1.國(guó)家重點(diǎn)研發(fā)計(jì)劃“發(fā)育編程及其代謝調(diào)節(jié)”項(xiàng)目，靈長(zhǎng)類原腸發(fā)育與器官形成的命運(yùn)決定調(diào)控機(jī)制，2021-2026，3100萬元

2.國(guó)家自然科學(xué)基金聯(lián)合基金項(xiàng)目，利用單堿基編輯技術(shù)構(gòu)建早衰癥猴模型及其機(jī)理研究，2022-2025，233萬元

3.國(guó)家自然科學(xué)基金面上項(xiàng)目，肌萎縮側(cè)索硬化癥靈長(zhǎng)類動(dòng)物轉(zhuǎn)基因模型的建立及早期發(fā)病機(jī)制研究，2021-2024，58萬元

六、聯(lián)系方式

地址：云南省昆明市呈貢區(qū)大學(xué)城景明南路727號(hào)昆明理工大學(xué)生命科學(xué)與技術(shù)學(xué)院

電話：0871-65952808

E-mail：niuyy@kust.edu.cn